Custom Service Inquiry Forms

What are you inquiring about today?

First Name

Last Name

Institutional/Company Email

example@example.com

Institution/Company

Telephone Number

Country

Cell Line Name and Species

Aim:

Species

Reporter

5'UTR of interest:

Do you need a control vector?

Which service would you prefer?

Will you supply the slides and/or antibody?

What type of sample will you provide?

Number of Samples

What is the antibody to be optimized?

How many antibodies?

Are you interested in H&E or Special Staining?

How many slides?

Are you interested in the Basic Service or the Complete Service?

Sample Submission Format

Services required:

Which CRISPR Bacterial Genome Editing Service do you prefer?

If "Other", please describe your project aim:

Titer Preferred

Will you send abm any starting materials?

Peptide Sequence

Target Protein Name

Target Antigen Information

Is antigen production (peptide format) required?

Quantity

Purity

Which expression system is preferred?

Which service is desired?

Type of samples to be submitted:

Services required for your samples:

Choose a service:

Total Number of Samples

Name of Target Gene

Which CRISPR gene or miRNA/lncRNA Deletion Service do you prefer?

Name of Target miRNA/lncRNA/Genomic Sequence

Name of Target Cell Line

Primer Application (e.g. PCR)

NCBI Accession Number:

Which system is preferred:

How many siRNAs are desired per target?

Is GFP needed?

Which Reporter Lentivector is preferred?

Which format is preferred:

Which selection cassette is preferred?

Is Vector or Virus preferred?

Is GFP or Luciferase preferred?

Promoter Name

Species of Cell Lines

Number of Cell Lines

Service preferred:

Option preferred:

Cat. No. of Cell Line

Select which coverage plan you would like:

Additional Comments/Questions

Brief description of project idea:

1. Goal of your Knock-in project:

2. Please provide information about your insert sequence.

3. Select your preferred deliverable for your Repair Template construct *Standard Deliverable: 1 μg of non-viral vector (pUC57) containing insert with left and right homology arm sequences

4. Please provide information about the target knock-in location.

5. Do you require Cas9 and/or sgRNA expression?

6. What CRISPR vector system would you prefer?

7. What is your preferred vector system for Cas9 and/or sgRNA expression?

8. Please provide information about sgRNAs.

9. Select your preferred deliverable for your Cas9 and/or sgRNA expression plasmids.

Select your preferred deliverable for your Cas9 and/or sgRNA expression plasmids. (Plasmid)

Select your preferred deliverable for your Cas9 and/or sgRNA expression plasmids. (Lentivirus)

10. Do you require any controls? abm offers a scrambled sgRNA control which does not target any mammalian gene.

11. Select your preferred deliverable for your scrambled control vector.

11-1. Select your preferred deliverable for your scrambled control vector. (Plasmid)

11-2. Select your preferred deliverable for your scrambled control vector. (Lentivirus)

1. Goal of your CRISPR experiment

Other

2. How many gene targets do you require?

Note: In order to initiate a quote you must know the total number of gene targets and must provide a complete list of gene names and NCBI accession number for all targets.

1. Name of gene or genetic information

2. Full sequence of gene or genetic element:

3. Will you be providing the insert DNA?

4. Desired vector backbone:

5. If 4a, please provide additional information:

6. If 4b, please provide additional information:

7. Select your preferred deliverable:

Select your preferred deliverable Plasmid DNA:

Select your preferred deliverable AAV Titer:

Select your preferred deliverable AAV Serotype:

1. Goal of your CRISPR experiment

2. Select preferred number of sgRNAs per vector

3. Select sgRNA sequences

Please list your 20nt sgRNA sequences

Please tell us 1) Gene name, 2) Gene species, and 3) Accession number.

4. Preferred deliverable

Preferred deliverable Plasmid DNA

Preferred deliverable Lentivirus

1. Goal of your CRISPR expression system

Other

2. Select preferred CRISPR expression system

3. Select preferred Cas9

4. How many sgRNA targets do your require

Other

5. Select sgRNA sequences

Please list your 20nt sgRNA sequences

Please tell us 1) Gene name, 2) Gene species, and 3) Accession number.

6. Select your preferred deliverable:

Select preferred deliverable Plasmid DNA

Select preferred deliverable AAV serotype at 10^11 GC/ml

8. Do you require any Controls? abm offers a scrambled sgRNA control which does not target any mammalian gene.

1. Goal of your CRISPR experiment

2. How many sgRNA targets do you require?

3. Select sgRNA sequences

Please list your 20nt sgRNA sequences

Please tell us 1) Gene name, 2) Gene species, and 3) Accession number.

4. Do you require Cas9 adenovirus?

5. Do you require any Controls? abm offers a scrambled sgRNA control which does not target any mammalian gene.

1. Goal of your CRISPR experiment

2. Select preferred CRISPR expression system

3. How many sgRNA targets do you require?

Other

4. Select sgRNA sequences

Please list your 20nt sgRNA sequences

Please tell us 1) Gene name, 2) Gene species, and 3) Accession number.

5. Select preferred deliverable Plasmid DNA

5. Select preferred deliverable

Select preferred deliverable Plasmid DNA

Select preferred deliverable Lentivirus

6. Do you require any Controls? abm offers a scrambled sgRNA control which does not target any mammalian gene.

1. Name of gene or genetic element:

2. Full sequence of gene or genetic element:

3. Desired vector backbone:

Please provide additional information:

4. Select your preferred deliverable:

1. Name of gene or genetic element:

2. Full sequence of gene or genetic element:

3. Will you be providing the insert DNA?

4. Desired vector backbone:

Please provide additional information:

5. Select your preferred deliverable:

Select your preferred deliverable Plasmid DNA

Select your preferred deliverable Lentivirus

1. How many samples require purification?

2. Required quantity of DNA per sample (mg)?

3. Plasmid copy number (low, medium, high):

4. Antibiotic resistance of your plasmid sample(s):

5. Do you require a specific E. coli strain to amplify your plasmid? If yes, please specify:

6. Do you require plasmid concentration normalization? If yes, please specify the desired final concentration.

1. Will you be sending abm any starting materials?

2. If no, please provide: 1) Previous order number, 2) Vector name, 3) List and identify the desired mutation(s) in your plasmid backbone sequence

3. If yes, please provide: 1) Name of your plasmid backbone(s), 2) Full sequence of your plasmid backbone(s), 3) List and identify the desired mutation(s) in your plasmid backbone sequence, 4) Plasmid antibiotic resistance, 5) Plasmid copy number

4. Select your preferred delierable:

Select Species:

1. List Species

2. Provide at least one circRNA identifier (Required)
• circAtlas ID blanks
• circBank ID blank
• circBase ID blanks
• Genomic Positionblank

3. Select your preferred deliverable

Additional Comments/Questions

Approximate Time Frame for Order Placement:

Reason for Requesting Quote:

How did you hear about us?

Nombre Completo

Institución o Laboratorio

País

Correo Electrónico

example@example.com

Número de Teléfono (Opcional)

Breve descripción del proceso de Investigación

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