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  • Methylcellulose Medium

    MethoCult™ H4034 Optimum

    MethoCult™ H4034 Optimum- 24 x 3 mL aliquots (Catalog #04044)

    Pre-aliquoted methylcellulose-based medium with recombinant cytokines for human cells. MethoCult™ H4034 Optimum has been formulated to support optimal growth of erythroid progenitor cells (BFU-E and CFU-E), granulocyte-macrophage progenitor cells (CFU-GM, CFU-G and CFU-M), and multipotential granulocyte, erythroid, macrophage, megakaryocyte progenitor cells (CFU-GEMM).

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  • Methylcellulose Medium

    MethoCult™ H4034 Optimum

    MethoCult™ H4034 Optimum- 100 mL bottle (Catalog #04034)

    Methylcellulose-based medium with recombinant cytokines for human cells. MethoCult™ H4034 Optimum has been formulated to support optimal growth of erythroid progenitor cells (BFU-E and CFU-E), granulocyte-macrophage progenitor cells (CFU-GM, CFU-G and CFU-M), and multipotential granulocyte, erythroid, macrophage, megakaryocyte progenitor cells (CFU-GEMM). 

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  • Methylcellulose Medium

    MethoCult™ H4435 Enriched

    MethoCult™ H4435 Enriched - 24 x 3 mL aliquots (Catalog #04435)

    Pre-aliquoted methylcellulose-based medium with recombinant cytokines for human cells. This medium supports optimal growth of erythroid progenitor cells (BFU-E and CFU-E), granulocyte-macrophage progenitor cells (CFU-GM, CFU-G and CFU-M), and multipotential granulocyte, erythroid, macrophage and megakaryocyte progenitor cells (CFU-GEMM).

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  • Methylcellulose Medium

    MethoCult™ H4435 Enriched

    MethoCult™ H4435 Enriched - 100 mL bottle (Catalog #04435)

    Methylcellulose-based medium with recombinant cytokines for human cells. This medium supports optimal growth of erythroid progenitor cells (BFU-E and CFU-E), granulocyte-macrophage progenitor cells (CFU-GM, CFU-G and CFU-M), and multipotential granulocyte, erythroid, macrophage and megakaryocyte progenitor cells (CFU-GEMM).

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  • Methylcellulose Medium

    MethoCult™ H4035 Optimum Without EPO

    MethoCult™ H4035 Optimum Without EPO - 100 mL bottle (Catalog #04035)

    Methylcellulose-based medium with recombinant cytokines (without erythropoietin [EPO]) for human cells. MethoCult™ H4035 Optimum Without is formulated to support optimal growth of granulocyte-macrophage progenitor cells (CFU-GM, CFU-G and CFU-M). The inclusion of G-CSF in Optimum formulations improves discrimination of myeloid colony subtypes.

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  • Methylcellulose Medium

    MethoCult™ H4535 Enriched Without EPO

    MethoCult™ H4535 Enriched Without EPO - 24 x 3 mL aliquots (Catalog #04545)

    Pre-aliquoted methylcellulose-based medium with recombinant cytokines (without erythropoietin [EPO]) for human cells. This medium supports optimal growth of granulocyte-macrophage progenitor cells (CFU-GM, CFU-G and CFU-M), and is recommended for use with CD34+ cells and other purified cell populations.

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  • Methylcellulose Medium

    MethoCult™ H4535 Enriched Without EPO

    MethoCult™ H4535 Enriched Without EPO - 100 mL bottle (Catalog #04535)

    Methylcellulose-based medium with recombinant cytokines (without erythropoietin [EPO]) for human cells. This medium supports optimal growth of granulocyte-macrophage progenitor cells (CFU-GM, CFU-G and CFU-M), and is recommended for use with CD34+ cells and other purified cell populations.

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  • Methylcellulose Medium

    MethoCult™ SF H4636

    MethoCult™ SF H4636 - 100 mL bottle (Catalog #04636)

    Serum-free methylcellulose-based medium with recombinant cytokines for human ES and iPS cell-derived cells. MethoCult™ SF H4636 is formulated to support optimal growth of erythroid progenitor cells (CFU-E and BFU-E), granulocyte-macrophage progenitor cells (CFU-GM, CFU-G, CFU-M), and multipotential progenitor cells (CFU-GEMM; granulocyte, erythrocyte, macrophage, megakaryocyte).

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  • Methylcellulose Medium

    MethoCult™ SF H4636

    MethoCult™ SF H4636 Without EPO - 100 mL bottle (Catalog #100-0945)

    Serum-free methylcellulose-based medium with recombinant cytokines for human hematopoietic cells. MethoCult™ SF H4636 Without EPO is a complete methylcellulose-based medium for the growth and enumeration of hematopoietic progenitor cells in colony-forming unit (CFU) assays of human bone marrow (BM), mobilized peripheral blood (MPB), peripheral blood (PB), and cord blood (CB) samples. MethoCult™ SF H4636 Without EPO is formulated to support the optimal growth of granulocyte-macrophage progenitor cells (CFU-GM, CFU-G and CFU-M). It is suitable for use with CD34+ enriched cells, mononuclear cells, and cells isolated by other purification methods.

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  • Incomplete MethoCult™ Media products: MethoCult™ M3234 (Catalog# 03234), MethoCult™ M3231 (Catalog# 03231), MethoCult™ SF M3236 (Catalog# 03236)*, Recombinant cytokines

    Product Catalog # Unit Size Description
    Mouse Recombinant G-CSF
    Human Recombinant G-CSF    		 78014
    78012

    100 µg
    100 µg

    		 For growth of granulocytic progenitor cells
    Mouse Recombinant GM-CSF 78017 100 µg For growth of granulocytic and monocytic progenitor
    cells
    Mouse Recombinant IL-3 78042 100 µg Used in combination with other cytokines to promote
    growth of early myeloid progenitor cells of all lineages
    Mouse Recombinant IL-6
    Human Recombinant IL-6    		 78052
    78050    		 100 µg
    100 µg    		 Pleiotropic cytokine for growth and differentiation of
    hematopoietic progenitor cells
    Mouse Recombinant M-CSF 78059 100 µg For growth of monocytic progenitor cells
    Mouse Recombinant SCF 78064 100 µg For growth of mast cells and used in combination with
    other cytokines to promote growth of myeloid and
    lymphoid progenitor cells
    Mouse Recombinant TPO
    Human Recombinant TPO    		 78072
    78210.1    		 50 µg
    100 µg
    • Used in combination with other cytokines to promote growth of megakaryocytic progenitor cells
    • Megakaryocytic progenitor cells are counted in MegaCult™-C collagen-based medium
    Human Recombinant EPO 78007 50 µg Used in combination with other cytokines for growth of
    erythroid progenitor cells

    *Incomplete MethoCult™ media allow researchers to add desired medium components, such as cytokines and growth factors, in order to prepare formulations for specific cell culture requirements.

  • Required Consumables

    • SmartDish™ (Catalog #27370, 27371)

    • 35 mm Culture Dishes (Catalog #27100, 27150)

    • Corning® 245 mm Square Dish, Non-Treated (Catalog #38020)

    • Syringe (3 mL or 6 mL; e.g. 3 cc Syringe, Catalog #28230, 28240)

    • Blunt-End Needles, 16 Gauge (Catalog #28110, 28120)

    • STEMgrid™-6 (Catalog #27000)

    • Iscove's MDM with 2% FBS (Catalog #07700)

    • Sterile D-PBS (Without Ca++ and Mg++; Catalog #37350)

    • Trypan Blue (Catalog #07050)

    • 3% Acetic Acid with Methylene Blue (Catalog #07060)

  • Required Consumables

    • SmartDish™ (Catalog #27370, 27371)

    • 35 mm Culture Dishes (Catalog #27100, 27150)

    • Corning® 245 mm Square Dish, Non-Treated (Catalog #38020)

    • Syringe (3 mL or 6 mL; e.g. 3 cc Syringe, Catalog #28230, 28240)

    • Blunt-End Needles, 16 Gauge (Catalog #28110, 28120)

    • STEMgrid™-6 (Catalog #27000)

    • Iscove's MDM with 2% FBS (Catalog #07700)

    • Sterile D-PBS (Without Ca++ and Mg++; Catalog #37350)

    • Trypan Blue (Catalog #07050)

    • 3% Acetic Acid with Methylene Blue (Catalog #07060)

  • Required Consumables

    • SmartDish™ (Catalog #27370, 27371)

    • 35 mm Culture Dishes (Catalog #27100, 27150)

    • Corning® 245 mm Square Dish, Non-Treated (Catalog #38020)

    • Syringe (3 mL or 6 mL; e.g. 3 cc Syringe, Catalog #28230, 28240)

    • Blunt-End Needles, 16 Gauge (Catalog #28110, 28120)

    • STEMgrid™-6 (Catalog #27000)

    • Falcon® Round-Bottom Tubes, 14 mL (Catalog #38008)

    • Iscove's MDM with 2% FBS (Catalog #07700)

    • Sterile D-PBS (Without Ca++ and Mg++; Catalog #37350)

    • Trypan Blue (Catalog #07050)

    • 3% Acetic Acid with Methylene Blue (Catalog #07060)

  • Related Resources

    Cell Culture

    Methods Library

    Explore our collection of protocols and tech tips for information and support on setting up, optimizing, and standardizing the CFU assay.

    Explore Now >

    Still have questions about our products?

    Talk to a sales representative about the products in your materials list, additional resources and products, or IVD formulations.

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  • Note: If you are participating in the On-Demand Hematopoietic Training Course, please note that the course contains materials and instructions specific to primary adult derived HSPCs. If you are working with PSC-derived CD34+ cells, you can find suggestions on plating concentration within the additional course resources.

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  • Methylcellulose Medium

    MethoCult™ GF M3434

    MethoCult™ GF M3434 (Catalog #03434)

    Methylcellulose-based medium with recombinant cytokines (including EPO) for mouse cells. MethoCult™ GF M3434 has been formulated to support optimal growth of primitive erythroid progenitor cells (BFU-E), granulocyte-macrophage progenitor cells (CFU-GM, CFU-G and CFU-M), and multi-potential granulocyte, erythroid, macrophage, megakaryocyte progenitor cells (CFU-GEMM).

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  • Methylcellulose Medium

    MethoCult™ GF M3534

    MethoCult™ SF M3534 (Catalog #03534)

    Methylcellulose-based medium with recombinant cytokines (without erythropoietin [EPO]) for mouse myeloid progenitor cells. MethoCult™ GF M3534 is optimized for the growth and enumeration of granulocyte-macrophage progenitor cells (CFU-GM, CFU-G, CFU-M) in colony-forming unit (CFU) assays of mouse bone marrow, spleen, peripheral blood, and fetal liver cells. MethoCult™ M3534 does not support the growth of erythroid progenitor cells (BFU-E and CFU-E) as it does not contain erythropoietin (EPO).

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  • Methylcellulose Medium

    MethoCult™ SF M3436

    MethoCult™ SF M3436 (Catalog #03436)

    Serum-free methylcellulose-based medium with recombinant cytokines (including EPO) for mouse erythroid progenitor cells. MethoCult™ SF M3436 is optimized for the growth and enumeration of primitive erythroid progenitor cells (BFU-E) in colony-forming unit (CFU) assays of mouse bone marrow, fetal liver, spleen, peripheral blood, and purified cells. MethoCult™ SF M3436 does not support the growth of granulocyte-macrophage progenitor cells (CFU-GM, CFU-G and CFU-M) or multipotential granulocyte, erythroid, macrophage, megakaryocyte progenitor cells (CFU-GEMM).

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  • Methylcellulose Medium

    MethoCult™ M3334

    MethoCult™ M3334 (Catalog #03334)

    Methylcellulose-based medium with EPO (without other cytokines) for mouse cells. MethoCult™ M3334 is optimized for the growth and enumeration of erythroid progenitor cells (late BFU-E and CFU-E) in colony-forming unit (CFU) assays of mouse bone marrow, spleen, and fetal liver cells. This formulation contains erythropoietin (EPO) but does not contain other recombinant cytokines.

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  • Methylcellulose Medium

    MethoCult™ M3630

    MethoCult™ M3630 (Catalog #03630)

    Methylcellulose-based medium for mouse pre-B lymphoid progenitor cells. MethoCult™ M3630 is optimized for the growth and enumeration of mouse pre-B lymphoid progenitor cells in colony-forming unit (CFU) assays of mouse bone marrow and Whitlock-Witte long-term cultures.

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  • Required support products for setting up the CFU Assay

    Products for Manual TNC and Viability Assessments

    • Trypan Blue (Catalog #07050)

    • 3% Acetic Acid with Methylene Blue (Catalog #07060)

    Required Consumables

    • SmartDish™ (Catalog #27370, 27371)

    • 35 mm Culture Dishes (Catalog #27100, 27150)

    • Corning® 245 mm Square Dish, Non-Treated (Catalog #38020)

    • Sterile round-bottom polystyrene tubes: 14 mL (17 x 95 mm; Catalog #38008)

    • Iscove's MDM with 2% FBS (Catalog #07700)

    • Sterile D-PBS (Without Ca++ and Mg++; Catalog #37350)

    • Syringe (3 mL or 6 mL; e.g. 3 cc Syringe, Catalog #28230, 28240)

    • Blunt-End Needles, 16 Gauge (Catalog #28110, 28120)

    • STEMgrid™-6 (Catalog #27000)

  • Explore below to learn more about required consumables for sample preparation:

    What tissues are you harvesting cells from?

    • Bone Marrow 
      • Forceps and scissors
      • Sterile Culture Dish eg. 35 mm Culture Dish (Catalog #27100) or larger 100 mm (Catalog #38045)
      • Syringe (1 mL)
      • 21 or 23 gauge needles
      • Sterile round-bottom polystyrene tubes: 5 mL (12 x 75 mm; Catalog #38007)
    • Peripheral Blood 
      • Syringes (1 mL)
      • 25 or 26 gauge needles
      • EDTA Microtainer® blood collection tubes
      • Red blood cell lysis buffer: Ammonium Chloride Solution (Catalog #07800 or #07850)
      • Sterile round-bottom polystyrene tubes: 14 mL (17 x 95 mm; Catalog #38008)
      • 1, 2, 5 or 10 mL serological pipette (e.g. Falcon® Serological Pipettes, 1 mL, Catalog #38001, Falcon® Serological Pipettes, 2 mL, Catalog #38002, Falcon® Serological Pipettes, 5 mL, Catalog #38003 or Falcon® Serological Pipettes, 10 mL, Catalog #38004)
    • Spleen 
      • Forceps and scissors
      • Sterile Culture Dish eg. 35 mm Culture Dish (Catalog #27100) or larger 100 mm (Catalog #38045)
      • Recommended dissociation medium according to your cell isolation kit's Product Information Sheet. Recommended options include:
        • EasySep™ Buffer (Catalog #20144)
        • RoboSep™ Buffer (Catalog #20104)
      • 3 cc Syringes (Catalog #28230)
      • 50 mL conical tube (e.g. Falcon® Conical Tubes, 50 mL, Catalog #38010)
      • 70 μm cell strainer (e.g. 70 µm Reversible Strainer, Large, Catalog #27260)
      • 5 or 10 mL serological pipette (e.g. Falcon® Serological Pipettes, 5 mL, Catalog #38003, or Falcon® Serological Pipettes, 10 mL, Catalog #38004)
    • End 
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