Molecular Diagnostics Technologist (MDT) AMT
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Minimum program requirements
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General Laboratory
Laboratory Safety
Reagents and Supplies
Safety Data Sheets (SDS)
HIPAA Compliance
Policies and Procedures
Basic Dilutions
Quality Assurance and Quality Control
Laboratory Equipment & Instrumentation Maintenance
General Molecular Diagnostics Theory
DNA/RNA
Basic Genetics
Endonucleases & Exonucleases
Proteins & Protein Expression
Pharmacogenomics
Oncology
Infectious Diseases (parasitic, viral, bacterial, fungal)
Molecular Laboratory Procedures
Sources of Carryover/Contamination, RNase-Free Environments
Workflow Importance, (unidirectional) test setup, and physical separation
Molecular & Biological Contaminants
QC metrics (quantitative vs. qualitative)
Proper Pipette Technique & Appropriate Volume Use for Each Pipette
Laboratory-Developed Tests & Steps Required to Validate and Report
Proficiency Testing
Regulatory Requirements
DNA and RNA Extraction Procedures
Specimen Preservation & Acceptability (e.g., ID, handling, storage, rejection criteria, aliquots)
Diagnostic Methods
Utility and Limitations of Molecular Diagnostic Assays
Primer Design (avoidance of cross-homology and template structure)
Primer Performance Characteristics
Fundamental Role of microRNA
Number & Type of Controls Necessary for Each Test (with acceptability criteria)
Cutoffs for Determination of Positive & Negative Tests
Reconstitute Reagents (master mix based on run size), Controls, Calibrators, Probes, Primers
Statistics of Positive & Negative Rates (contamination rates, test performance, inhibition)
Standard Curve and Establishing Linearity
Fundamental Role of microRNA
Importance of Housekeeping Genes
Query and Cite Mutation References
Calibrations for Quantitative Tests (HBV, HIV)
Gas Chromatography-Mass Spectrometry (GC-MS)
Polymerase Chain Reaction
Assays
Sequencing
Microarrays
FISH
Electrophoresis
DNA/RNA Extraction
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