Imaging Facility Training Requisition Form
To proceed with training, please complete this form so that your imaging experience and experimental needs can be assessed. Once returned, the imaging specialist will contact you to book a consultation. Fill out a new form for each new experimental aim.
Trainee/Staff Information
Name
*
First Name
Last Name
Position
*
Email
*
example@example.com
Lab
*
Are you a research visitor or student that will be working here for 6 months or less?
Yes
No
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Previous Training and Experience
Identify all imaging systems which you have been previously trained to use. (Check all that apply.)
Basic training - Olympus BX50
Zeiss Wide Field with Deconvolution
Zeiss Live Cell with FLIM
Quorum Spinning Disc
Quorum Spinning Disc with TIRF
Nikon A1R with Multiphoton
Biotek Cytation 5
Zeiss Cell Discoverer 7
Other
List the imaging systems you have experince with.
*
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Sample Type
Select your sample type.
*
Cell Monolayer
Tissue
Organoid or Organism
Other
Tissue Thickness (in um)
*
Tissue Preparation
*
FFPE
Cryo-section
Describe your sample including dimensions and thickness (in um).
*
Describe the type of organoid or organism and its dimensions including thickness (in um).
*
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Sample Format
Reminder that glass coverslips or glass bottom dishes and plates should be 0.17mm thick (#1.5).
Select your sample format.
*
Cover slip - mounted on slide
Cover slip - unmounted
35mm glass bottom dish
Multi-well plate
Chamber slide or Ibidi slide
Other
Multiwell plate type.
*
6-well
12-well
24-well
48 well
96 well
Multiwell plate base.
*
Plastic bottom
Glass bottom
Provide details of your sample format.
*
Provide chamber slide or ibidi slide details.
*
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Safety
Does your sample contain any hazardous material (biological and/or chemical)?
*
Yes
No
Indicate the risk group of your sample/pathogen.
*
RG1
RG2
Indicate if the pathogen is in your lab’s biosafety permit.
*
Yes
No
Describe hazardous materials (biological and/or chemical).
*
Any samples (cells, tissues, organisms etc.) brought into the imaging facilities should follow all biosafety and vivarium safety protocols. Any chemical hazards should follow appropriate containment and disposal protocols. All surfaces in contact with cells, tissues, or animals should be cleaned and dried. Gloves from your lab should not be brought into imaging facilities although you may put on clean ones.
*
Acknowledged
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Live Cell Imaging
Is your sample fixed or living?
*
Fixed
Living
Indicate incubation requirements
*
Temperture
Humidity
Gas - CO2
Gas - Hypoxia
For multiple timepoints, indicate the frame rate.
*
Faster than 10 frames per second
Seconds
Minutes
Hours
Days
Not applicable - i.e. not imaging more than 1 timepoint
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Fluorescence Requirements
Indicate all fluorophores and/or fluorescent proteins in your sample.
*
UV excitable - Alexa 405, DAPI, Hoechst 33258, etc.
Violet excitable - Alexa 430, eCFP, Lucifer Yellow, etc.
Blue excitatiable - Alexa 488, eGFP, Fluo4, FITC, etc.
Cyan excitatiable - Alexa 514, eYFP, Eosin, TOTO-1 etc.
Green excitatable - Alexa 555, Cy3, TRITC, DsRed, etc.
Yellow excitatable - Alex 568, Alexa 594, mCherry, mPlum, etc.
Red excitable - Alexa 631, Alexa 647, Cy5, TOTO-3, etc.
Other
Provide excitation and emission characteristics. (These can be found with online spectral viewers or on product information pages.)
*
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Transmitted Light Requirements
Indicate transmitted light requirements
*
Bright field
Colour bright field (e.g. for H&E)
Phase Contrast
DIC
None
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Resolution Requirements
What is the required spatial resolution?
*
200nm -500nm (e.g. centrosomes)
500nm - 1um (e.g. small organelles)
1um - 5um (e.g. large organelles, platelets etc.)
5um -20um (e.g. nuclei)
> 20um (e.g. cells)
Unsure
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Experimental Aim and Analysis Requirements
Briefly describe your experimental and analysis aims.
*
Indicate your technical and experimental controls.
*
Analysis training will only be provided for data sets with the appropriate technical and experimental controls.
*
Acknowledged
Identify image analysis software that you are experienced with. (Check all that apply.)
Fiji
QuPath
Cell Profiler
Imaris
Halo
Volocity
NIS Elements
Leica LAS
Zen
Metamorph
Gen5
MetaXpress
Other
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Acknowledgements
Completion of online training modules as well as basic training in wide-field microscopy is a prerequisite for advanced training.
*
Acknowledged
The imaging facility is not responsible for lost data. It is the user's responsibility to back up raw data and record all processing and analysis steps.
*
Acknowledged
Misuse of imaging facility systems may result in loss of access and/or fees for cleaning and repair of damaged equipment as necessary.
*
Acknowledged
If the Core Specialists have provided substantial contributions to the conception or design of the work; or the acquisition, analysis, or interpretation of data for the work, please include them in the authorship list.
*
Acknowledged
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