• Which application best describes your assay?*
  • Are you performing a PCR melt curve analysis?*
  • Does your target require enhanced specificity (i.e. SNP deletion, allelic discrimination or differentiation of target from pseudogene)?*
  • Do you anticipate your probe sequence to be longer than 25 bases such as in an AT-rich target sequence?*
  • Which is more important?*

  • We recommend:

    Molecular beacons

    or

    Scorpions primers

     

  • We recommend:

    BHQ Probes

     

  • We recommend:

    BHQnova - a probe that is longer than 25 bases and typically AT-rich to reach the desired hydrolysis probe Tm

     

  • We recommend: 

    Locked nucleic acid (LNA) probes

     

     

  • We recommend: 

    BHQplus

    or 

    Minor groove binder (MGB) probes

     

  • Should be Empty: