Which probe is best for your assay? v3

Which application best describes your assay?*

Gene expressionCopy number variationPresence / absencePathogen detection / viral load quantificationInsertion / deletionSNP genotyping / allelic discriminationPolyploid genotyping (plants)

Are you performing a PCR melt curve analysis?*

YesNo

Does your target require enhanced specificity (i.e. SNP deletion, allelic discrimination or differentiation of target from pseudogene)?*

YesNo

Do you anticipate your probe sequence to be longer than 25 bases such as in an AT-rich target sequence?*

YesNo

Which is more important?*

Finely tuned Tm or exclude pseudogene detectionSimplicity in probe design and implementation

We recommend:

Molecular beacons

or

Scorpions primers

We recommend:

BHQ Probes

We recommend:

BHQnova - a probe that is longer than 25 bases and typically AT-rich to reach the desired hydrolysis probe Tm

We recommend:

Locked nucleic acid (LNA) probes

We recommend:

BHQplus

or

Minor groove binder (MGB) probes

Should be Empty: