• NUCLEICA Form

    If you are interested in a CRISPR screen or a heavily customizable project, please do not use this form and contact us directly. If you are looking for a knockout, knockin or a deletion, please proceed as follows:

  •  -

  • Service required

  • Please, select our service (s)*
  • Outsourcing - If you are interested in other outsourcing services, please select the services you want and fill out the form below.*

  •  Knock-out service description

    Clones Bulk
    • 2 “knocked-out” clones (both alleles) made with a single gRNA and 2 clones from the same pool of transfected cells that did not edit either allele;
    • Test for mycoplasma;
    • Strategy design and testing of up to 4 gRNAs;
    • Electroporation of RNPs and GFP-encoding plasmid;
    • Sorting of GFP+ cells;
    • Confirmation by Sanger sequencing that the locus is being targeted by the Cas9 endonuclease;
    • Clone isolation;
    • Clone genotyping and knockout confirmation by Sanger sequencing;
    • Maintenance of edited clones in our collection for six months;
    • Clones can be resent free of charge (excluding shipping costs).
    • 2 “knocked-out” bulk cultures made with a single gRNA;
    • Test for mycoplasma;
    • Strategy design and testing of up to 4 gRNAs;
    • Electroporation of RNPs and GFP-encoding plasmid;
    • Sorting of GFP+ cells;
    • Confirmation by Sanger sequencing that the locus is being targeted by the Cas9 endonuclease;
    • Maintenance of edited cells in our collection for six months;
    • Cultures can be resent free of charge (excluding shipping costs).
  • Please, select required knock-out service
  • Please, select number of differents cell lines to edit - knock-out clones
  • Please, select number of differents cell lines to edit - knock-out bulk
  • Knock-out clone for one cell line
    Rows
  • Knock-out clones for two cell lines
    Rows
  • Knock-out clones for three cell lines
    Rows
  • Knock-out clones for four cell lines
    Rows
  • Please, if you choose "Other" for medium field, use this box to indicate the medium, a special type of FBS, the % of FBS if different from 10%, etc. Use commas to separate these items.
  • * Please indicate any special supplement. It is not necessary to include Pen/strep, sodium pyruvate, gluatamine, HEPES or b-mercaptoethanol.
    ** If you want to knockout more than one gene, introduce the gene abbreviations separated by commas. For instance: "geneA, geneB, geneC."
    ***If you know the karyotype of the cell line, type the number of alleles of each gene separated by commas. For instance: "2, 3, 4" for a cell line with 2, 3, and 4 alleles for geneA, geneB, and geneC, respectively.
    **** By default, homozygous clones for the knock-out are always provided. Sometimes, it may be useful to study heterozygous (+/-) clones as well. If that's the case for at least one of your genes, please indicate it using commas to separate the different answers if you have more than one gene. For instance: "Yes, No, Yes" indicates that you also want heterozygous clones for the A and C knock-outs but not the B knock-out.
    ***** If you indicated more than one gene, we will assume that you want independent clones, one for each knock-out. If you also want a clone combining two (a double knock-out) or more knock-outs, please use this column to indicate the knock-out combinations. If you want more than one combination, please use commas to separate them. For instance: "geneA+geneB, geneB+geneC, geneA+geneB+geneC" indicates that besides the single knock-outs, you also want two double knock-outs (the AB and BC pairs) and a triple knock-outs (the ABC combination).

     

  • Knock-out bulk for one cell line
    Rows
  • Knock-out bulks for two cell lines
    Rows
  • Knock-out bulks for three cell lines
    Rows
  • Knock-out bulks for four cell lines
    Rows
  • Please, if you choose "Other" for medium field, use this box to indicate the medium, a special type of FBS, the % of FBS if different from 10%, etc. Use commas to separate these items.
  • * Please indicate any special supplement. It is not necessary to include Pen/strep, sodium pyruvate, gluatamine, HEPES or b-mercaptoethanol.
    ** If you want to knockout more than one gene, introduce the gene abbreviations separated by commas. For instance: "geneA, geneB, geneC."
    ***If you know the karyotype of the cell line, type the number of alleles of each gene separated by commas. For instance: "2, 3, 4" for a cell line with 2, 3, and 4 alleles for geneA, geneB, and geneC, respectively.
    **** If you indicated more than one gene, we will assume that you want independent clones, one for each knock-out. If you also want a clone combining two (a double knock-out) or more knock-outs, please use this column to indicate the knock-out combinations. If you want more than one combination, please use commas to separate them. For instance: "geneA+geneB, geneB+geneC, geneA+geneB+geneC" indicates that besides the single knock-outs, you also want two double knock-outs (the AB and BC pairs) and a triple knock-outs (the ABC combination).

     

  • Knock-in service description

    Clones Bulk
    • 2 “knocked-in" clones (both alleles) made with a single gRNA and 2 clones from the same pool of transfected cells that did not edit either allele;
    • Test for mycoplasma;
    • Strategy design and testing of up to 4 gRNAs;
    • SNP or small tag template;
    • Electroporation of RNPs and GFP-encoding plasmid;
    • Sorting of GFP+ cells;
    • Confirmation by Sanger sequencing that the locus is being targeted by the Cas9 endonuclease;
    • Clone isolation;
    • Clone genotyping confirmation by Sanger sequencing;
    • Maintenance of edited clones in our collection for six months;
    • Clones can be resent free of charge (excluding shipping costs).
    • 2 “knocked-in” bulk cultures;
    • Test for mycoplasma;
    • Strategy design and testing of up to 4 gRNAs;
    • SNP or small tag template;
    • Electroporation of RNPs and GFP-encoding plasmid;
    • Sorting of GFP+ cells;
    • Confirmation by Sanger sequencing that the locus is being targeted by the Cas9 endonuclease;
    • Maintenance of edited clones in our collection for six months;
    • Cultures can be resent free of charge (excluding shipping costs).
  • Please, select required Knock-in service
  • Please, select number of differents cell lines to edit - knock-in clones
  • Please, select number of differents cell lines to edit - knock-in bulk
  • Knock-in clone for one cell line
    Rows
  • Knock-in clones for two cell lines
    Rows
  • Knock-in clones for three cell lines
    Rows
  • Knock-in clones for four cell lines
    Rows
  • Please, if you choose "Other" for medium field, use this box to indicate the medium, a special type of FBS, the % of FBS if different from 10%, etc. Use commas to separate these items.

  • * Please indicate any special supplement. It is not necessary to include Pen/strep, sodium pyruvate, gluatamine, HEPES or b-mercaptoethanol.
    ** If you want to knockin more than one gene, introduce the gene abbreviations separated by commas. For instance: "geneA, geneB, geneC."
    ***If you know the karyotype of the cell line, type the number of alleles of each gene separated by commas. For instance: "2, 3, 4" for a cell line with 2, 3, and 4 alleles for geneA, geneB, and geneC, respectively.
    **** Using commas to separate the different answers, please indicate whether you want the knock-in to be homozygous (homoz) or heterozygous (het). For instance: "homoz, het, homoz" indicates that you want homozygous clones for the A and C knock-ins but not the B knock-in.
    ***** Please indicate the type of knock-in for each edition, separated by commas. Use the following legend: 

    Triplet or SNP: the modification of a single DNA triplet encoding an aminoacid (typical modification to replace aminoacids)

    Small TAG: insertion of a tag under 150 base pairs (for instance, a FLAG tag).

    Large TAG: an insertion above 150 base pairs (for instance, the insertion of a GFP gene).

    ****** If you indicated more than one gene, we will assume that you want independent clones, one for each knock-in. If you also want a clone combining two or more knock-ins, please use this column to indicate the knock-in combinations. If you want more than one combination, please use commas to separate them. For instance: "geneA+geneB, geneB+geneC, geneA+geneB+geneC" indicates that besides the single knock-ins, you also want two double knock-ins (the AB and BC pairs) and a triple knock-in (the ABC combination).

     

  • Knock-in bulk for one cell line
    Rows
  • Knock-in bulk for two cell lines
    Rows
  • Knock-in bulk for three cell lines
    Rows
  • Knock-in bulk for four cell lines
    Rows
  • Please, if you choose "Other" for medium field, use this box to indicate the medium, a special type of FBS, the % of FBS if different from 10%, etc. Use commas to separate these items.

  • * Please indicate any special supplement. It is not necessary to include Pen/strep, sodium pyruvate, gluatamine, HEPES or b-mercaptoethanol.
    ** If you want to knockin more than one gene, introduce the gene abbreviations separated by commas. For instance: "geneA, geneB, geneC."
    ***If you know the karyotype of the cell line, type the number of alleles of each gene separated by commas. For instance: "2, 3, 4" for a cell line with 2, 3, and 4 alleles for geneA, geneB, and geneC, respectively.
    **** Please indicate the type of knock-in for each edition, separated by commas. Use the following legend: 

    Triplet or SNP: the modification of a single DNA triplet encoding an aminoacid (typical modification to replace aminoacids)

    Small TAG: insertion of a tag under 150 base pairs (for instance, a FLAG tag).

    Large TAG: an insertion above 150 base pairs (for instance, the insertion of a GFP gene).

    ***** If you indicated more than one gene, we will assume that you want independent clones, one for each knock-in. If you also want a clone combining two or more knock-ins, please use this column to indicate the knock-in combinations. If you want more than one combination, please use commas to separate them. For instance: "geneA+geneB, geneB+geneC, geneA+geneB+geneC" indicates that besides the single knock-ins, you also want two double knock-ins (the AB and BC pairs) and a triple knock-in (the ABC combination).

  • Deletion service description

    Clones Bulk
    • 2 “deletion” clones (both or single alleles as requested) made with one pair of gRNAs and 2 clones from the same pool of transfected cells that did not edit either allele;
    • Test for mycoplasma;
    • Strategy design and testing of up to 4 gRNAs;
    • Electroporation of RNPs and GFP-encoding plasmid;
    • Sorting of GFP+ cells;
    • Confirmation by Sanger sequencing that the locus is being targeted by the Cas9 endonuclease;
    • Clone isolation;
    • Clone genotyping and delection confirmation by Sanger sequencing;
    • Maintenance of edited clones in our collection for six months;
    • Clones can be resent free of charge (excluding shipping costs).
    • 2 “deletion” bulk cultures;
    • Test for mycoplasma;
    • Strategy design and testing of up to 4 gRNAs;
    • Electroporation of RNPs and GFP-encoding plasmid;
    • Sorting of GFP+ cells;
    • Confirmation by Sanger sequencing that the locus is being targeted by the Cas9 endonuclease;
    • Maintenance of edited clones in our collection for six months;
    • Cultures can be resent free of charge (excluding shipping costs).
  • Please, select required Deletion service
  • Please, select number of differents cell lines to edit - deletion clones
  • Please, select number of differents cell lines to edit - deletion bulk
  • Deletion clone for one cell line
    Rows
  • Deletion clones for two cell lines
    Rows
  • Deletion clones for three cell lines
    Rows
  • Deletion clones for four cell lines
    Rows
  • Please, if you choose "Other" for medium field, use this box to indicate the medium, a special type of FBS, the % of FBS if different from 10%, etc. Use commas to separate these items.

  • * Please indicate any special supplement. It is not necessary to include Pen/strep, sodium pyruvate, gluatamine, HEPES or b-mercaptoethanol.

    ** If you want  more than one deletion, introduce the gene abbreviations of the locus of the deletion separated by commas. For instance: "geneA, geneB, geneC." If you have more than one deletion in the same locus, please write "geneA1, geneA2...).

    ***If you know the karyotype of the cell line, type the number of alleles of each target gene separated by commas. For instance: "2, 3, 4" for a cell line with 2, 3, and 4 alleles for geneA, geneB, and geneC, respectively.

    **** By default, homozygous clones for the deletion are always provided. Sometimes, it may be useful to study heterozygous (+/del) clones as well. If that's the case for at least one of your genes, please indicate it using commas to separate the different answers if you have more than one gene. For instance: "Yes, No, Yes" indicates that you want heterozygous clones for the geneA and geneC deletion but not the geneB deletion.

    ***** Please indicate the approximate size of the deletion. If you indicated more than one deletion, separate the different sizes using commas.

    ****** If you indicated more than one deletion, we will assume that you want independent clones, one for each deletion. If you also want a clone combining two or more deletions, please use this column to indicate the deletion combinations. If you want more than one combination, please use commas to separate them. For instance: "geneA+geneB, geneB+geneC, geneA+geneB+geneC" indicates that besides the single deletion, you also want two double deletion (the AB and BC pairs) and a triple deletion (the ABC combination).
  • Deletion bulk for one cell line
    Rows
  • Deletion bulk for two cell lines
    Rows
  • Deletion bulk for three cell lines
    Rows
  • Deletion bulk for four cell lines
    Rows
  • Please, if you choose "Other" for medium field, use this box to indicate the medium, a special type of FBS, the % of FBS if different from 10%, etc. Use commas to separate these items.

  • * Please indicate any special supplement. It is not necessary to include Pen/strep, sodium pyruvate, gluatamine, HEPES or b-mercaptoethanol.

    ** If you want  more than one deletion, introduce the gene abbreviations of the locus of the deletion separated by commas. For instance: "geneA, geneB, geneC." If you have more than one deletion in the same locus, please write "geneA1, geneA2...).

    ***If you know the karyotype of the cell line, type the number of alleles of each target gene separated by commas. For instance: "2, 3, 4" for a cell line with 2, 3, and 4 alleles for geneA, geneB, and geneC, respectively.

    **** Please indicate the approximate size of the deletion. If you indicated more than one deletion, separate the different sizes using commas.

    ***** If you indicated more than one deletion, we will assume that you want independent clones, one for each deletion. If you also want a clone combining two or more deletions, please use this column to indicate the deletion combinations. If you want more than one combination, please use commas to separate them. For instance: "geneA+geneB, geneB+geneC, geneA+geneB+geneC" indicates that besides the single deletion, you also want two double deletion (the AB and BC pairs) and a triple deletion (the ABC combination).

  • Do it yourself description

    "Do it yourself" is a kit for 4 reactions, consisting of:

    • cells;
    • gRNA;
    • genotyping primers;
    • templates;
    • Cas9.

    All kits are developed by our researchers based on the specifications of each project.
    All "Do it yourself" kits are accompanied by online consulting for 6 months.

  • Please, select the service (s)

  • Knockdown

    Knockdowns are made with lentiviruses expressing shRNAs against the target RNA.

  • Gene activation

    Gene expression is activated using CRISPR tools.

  • Customized lenti and retroviruses

    Lenti and retroviruses encoding specific sequences are delivered as frozen supernatants with a high viral titter and/or as plasmids to be trasfected.

  • Customized plasmids

    Plamids are built using a combination of subcloning, PCR and sinthesized sequences.

  • Extra services

    GENETAGUS can provide extra services to improve your project. Please, select the required service below.
  • Extra services
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