Transformation Request Form

1

After we provide the transgenic lines, please let us know the results of the verification. The feedback is helpful!

*Seeds/plants/plant material will need to be provided in the following situations:

1. Seedling material (e.g. cotyledons and hypocoyls) is the target for transformation.

For tomato transformation experiments, 1 g of seed is needed for each transformation request.

2. Our facility does not have a readily available supply of material for the crop of interest.

2

Project Leader* This field is required.

3

Requestor* This field is required.

4

Email* This field is required.

example@example.com

5

Plant and genotype* This field is required.

6

What is the expected phenotype?* This field is required.

7

Are there any potential negative effects expected?* This field is required.

8

Construct name (use one form submission for each request)* This field is required.

9

Please provide the name of the vector you used* This field is required.

10

Please attach a plasmid map/diagram

Drag and drop files here

Select files to upload

Max. file size: 10.6MB

11

What method was used to verify the integrity of the construct?* This field is required.

12

What is the concentration of the plasmid DNA (5 ul of plasmid at 50 - 200 ng/ul in water is needed)?* This field is required.

13

Bacterial selection* This field is required.

14

Plant selection (antibiotic, herbicide component, etc.)* This field is required.

15

Number of transformed plants needed* This field is required.

16

Type of Vector* This field is required.

Knock-Down

Over-Expression

17

Vector-only controls needed?* This field is required.

Yes

No

18

If vector-only controls are needed, please provide vector-only construct in an attachment

Drag and drop files here

Select files to upload

Max. file size: 10.6MB

19

Number of vector-only control plants needed

20

Regenerated controls (no transformation) needed?* This field is required.

Yes

No

21

Number Needed* This field is required.

22

Any special requests or pertinent information?* This field is required.