Products
abm offers a genome-wide collection of human, mouse, and rat genes in mammalian protein vectors driven by a CMV promoter.
See below for the full list of purification tags available:
TAG |
TERMINAL |
CLEAVABLE? |
His |
C-terminal |
No |
HA |
C-terminal |
No |
D tag + HA |
N-terminal (D tag), C-terminal (HA) |
Yes (D tag), No (HA) |
D tag + His |
N-terminal (D tag), C-terminal (His) |
Yes (D tag), No (His) |
More Details
Protein vectors are a non-viral option for expressing protein to be purified. Protein vectors include a purification tag that will be fused to the gene of interest, making it easy to purify the protein once it is expressed. To prevent potential interference of the purification tag with normal protein function, some tags are able to be cleaved from the protein of interest using proteolytic agents such as thrombin or TEV protease.
Purification tags may be fused to either the C- or N-terminal of a protein, and can work in a variety of ways. Affinity tags allow for proteins to be purified using an affinity technique. Examples include maltose binding protein (MBP), glutathione-S-transferase (GST), and His tags. Epitope tags can be used to purify proteins using high-affinity antibodies which bind to them. Examples include HA tags, and FLAG tags (D tags). As well, certain tags may help the protein of interest to fold correctly and keep it from precipitating. These are called solubilization tags, and include MBP and GST.
When a mammalian gene is translated in mammalian cells, it will undergo all post-translational modifications that would occur in nature. Therefore protein produced in a mammalian system will be more authentic for experimental use than one produced in a bacterial system. However, mammalian cells are much more difficult and time-consuming to culture than bacterial cells. So, for applications where post-translational processing is important, mammalian expression is a good choice. If large amounts of post-translationally processed protein are required, a baculoviral expression system should be considered.
For a transgene to be expressed in mammalian cells, it must be driven by a mammalian promoter. Common mammalian promoters that are used in protein vectors include CMV, EF1a, CAG, and EF1a.