Trainings Offered
We offer the following trainings, limited to 4 individuals at a time.
Tufts CMS Orientation Tour
Tufts CMS staff provides investigators a tour of the animal facility, including information on locations of necessary supplies and equipment, use of procedure rooms, security access, contacts for specific needs and issues, and proper attire and personal protective equipment requirements for animal rooms.
Rodents 101 Wet Lab - Handling, restraint, identification, and euthanasia
Tufts CMS staff provides investigators hands-on training in this introductory wet lab on rodent handling, restraint, identification, and euthanasia. Investigators are provided opportunities to perform these techniques under the trainer’s supervision.
Rodent 102 Wet Lab – Drug Administration (PO, IP, IV, SQ)
Intermediate-level workshop
Prerequisite: Given rodent restraint is paramount to successful drug administration, Rodents 101 Animal Handling and/or must be proficient in manual animal handling and restraint.
Tufts CMS staff provides investigators hands-on training in this intermediate-level wet lab on drug administration using the following common routes: intraperitoneal (IP), subcutaneous (SQ), intravenous (IV), and oral gavage (PO). Investigators are provided opportunities to perform these techniques under the trainer’s supervision.
Rodents 103 Wet Lab – Blood Collection (Tail vein, Submandibular/Facial Vein)
Intermediate-level workshop on common blood collection techniques (e.g., tail vein, facial vein).
Prerequisite: Given rodent restraint is paramount to successful blood draw, Rodents 101 Animal Handling and/or be proficient in manual animal handling and restraint.
Tufts CMS staff provides investigators hands-on training in this intermediate-level wet lab on blood collection from the following locations: tail vein puncture, tail snip, submandibular technique, and cardiac puncture. Investigators are provided opportunities to perform these techniques under the trainer’s supervision.
Rodents 201 Wet Lab – Surgery
Advanced-level workshop on surgical preparation, technique, and post-operative care
Tufts CMS staff provides investigators hands-on training in this advanced-level wet lab on performing surgeries on rodents. During this training, investigators will learn about the importance of aseptic technique; providing adequate care during pre-surgery, surgery, and post-surgery periods; and basic nursing care to post-surgical animals. Investigators are provided opportunities to perform surgeries on euthanized rodents. This training is strongly recommended for anyone who is or will be performing survival surgery on rodents.
Bench Top Anesthesia Workshop
Tufts CMS staff provides investigators hands on-training in this introductory wet lab on how to operate the isoflurane anesthesia machines in Tufts CMS animal facilities. Training on these machines is mandatory before using them in the facility.
Mouse Necropsy Workshop
Tufts CMS staff provides investigators hands on-training on performing a basic mouse necropsy, including appropriate tissue handling techniques, normal mouse anatomy, tissue collection procedures, and basic pathology documentation.
Histology Prep Workshop
Tufts CMS staff provides investigators hands-on training on the proper methods to prepare mouse and rat tissues when submitting them to the Animal Histology Core in Tufts CMS, including fixation, tissue trimming, decalcification, tissue orientation, and cassetting. During the training, trimming of tissues will be demonstrated. Investigators are provided opportunities to trim tissues under the trainer’s supervision. Participants are welcome to bring fixed tissues that they work with in their laboratory.
Rodent Breeding and Transnetyx Colony Software Use
The CMS Rodent Breeding Service (RBS) offers information or individualized trainings for breeding of mice and rats and the use of Transnetyx for genotyping and colony maintenance. For training, please contact TuftsCMS-rodent-breeding@elist.tufts.edu
Investigators will be informed about Tufts CMS’ Rodent Breeding Services, which is designed to aid investigators in the creation, propagation, and maintenance of rodent breeding colonies. Services may include, but are not limited to, mating, weaning, collection of samples for DNA analysis, and record keeping.
We can also provide basic training in the use of Transnetyx services for genotyping and colony maintenance or can connect you with Transnetyx for personalized training.
Zebrafish 101
Tufts CMS staff provides investigators hands-on training in this introductory wet lab on basic information for working with zebrafish at Tufts. This includes an orientation to the zebrafish facility, where to find the appropriate supplies, learning about the facility’s procedures and protocols, and contacts for specific needs or issues. Our Zebrafish Facility Coordinator will train you on how to properly remove or add tanks on and off the system, how to handle fish, how to move fish from one tank to another, and how to anesthetize and euthanize zebrafish. Investigators are provided the opportunity to perform these techniques under the trainer’s supervision.
Private Training Sessions
We offer several individualized trainings for specialized surgical and non-surgical techniques not covered in the group trainings. These trainings are offered for both rodent and non-rodent models. Tufts CMS staff will train you to perform protocol specific techniques. Fees are based on an hourly rate, but may also include additional charges for specialized supplies, animals, etc. not covered in our standard training cost.