OSU SARL Zebrafish/Services Order Form

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Oregon State University - SARL

Zebrafish and Services Order Form
GENERAL INFORMATION

OSU ZBR and SARL provides services to both internal and external users of Oregon State University. The discount is listed below in the "Ordering" section of this form.

Services include fish, fish care, toxicity and behavior testing.

ZEBRAFISH BACKGROUND INFORMATION

The SPF monitored AB and 5D Tropical lines were originally screened for Pseudoloma neurophilia using qRTPCR (Sanders, 2011). After entering the SPF facility all lines are routinely screened for Pseudoloma neurophilia and other pathogens of concern (Kent, 2011).Since their introduction into the SARL facility, the lines have been maintained using a method that is in accordance with the ZIRC's protocol for AB and wild type next generation propagation. Each new generation is derived from the offspring of no less than 25 small group crosses, containing 3 males and up to 3 females each. Equal numbers of contributors are pulled from each cross to create the next generation. Embryos are screened daily until 5 dpf for the morphological defect.

Sanders JL, Kent ML (2011) Development of a sensitive assay for the detection of Pseudoloma neurophilia in laboratory ¬populations of the zebrafish Danio rerio. Dis Aquat Org 96:145-156

Kent ML, Buchner C, Watral VG, Sanders JL, LaDu J, Peterson TS, Tanguay RL (2011) Development and maintenance of a specific pathogen-free (SPF) zebrafish research facility for Pseudoloma neurophilia. Dis Aquat Org 95:73-79
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If you are an OSU user, please use this form.
Self Collection will be at Sinnhuber Aquatic Research Laboratory located at 28645 E Hwy 34, Corvallis, OR 97333. Do not arrive unless you receive a confirmation. Orders less than 72 hours notice will not be fulfilled.
ZBR is currently only providing delivery to a locations within the United States. Shipping is typically through FedEx Overnight (Priority). If other accomondations is needed, please indicate in the notes box when placing an order.

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The building is locked at all times. Please select a date and time to ensure we will have your order ready by then. Do not proceed without a confirmation email from the ZBR.
Date for Pick Up

Will we be shipping this domestically (within the US)
Shipments are sent only on Tuesday and Wednesday. Select a two week window of when you would like to take delivery (Wednesday or Thursday).

Note: Embryos sent will be bleached and provided in embryo medium + methylene blue [an antifungal]. Upon arrival, the embryos will be ~48 hours post-fertilization (hpf).
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Request/Comments

Zebrafish:

There is a maximum of 10 pairs of adult zebrafish per order (regardless of strain). Embryos are available in multiples of 100.

Services:

Make sure to uncheck "Shipping, Supplies and Handling"
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Order Placement

Animals

Adult Zebrafish

Cost for a pair of fish

$386.00

Strain	Quantity	Item #
AB [Wildtype]
5D [Wildtype]
Tg(sox9b:EGFP)uw101Tg [Transgenic:Tanguay]
casper a9/w2 [Transgenic: need MTA]
Tg(fli1a:EGFP)y1 [Transgenic: need MTA]
Tg(fabp10a:CFP-NTR)S891 [Transgenic: need MTA]
TgBAC(cyp1a:nls-egfp)cms2Tg [Transgenic: need MTA]

Zebrafish Embryos

Quantity is per 100 embryos. Other zebrafish lines may be available with a Material Transfer Agreement (MTA) with the originating lab. Only select this option after consulting with the ZBR.

$386.00

Strain	Quantity	Item #
AB [Wildtype]
5D [Wildtype]
Tg(sox9b:EGFP)uw101Tg [Transgenic:Tanguay]
Tg(fli1a:EGFP)y1 [Transgenic: need MTA]
casper a9/w2 [Transgenic: need MTA]
Tg(fabp10a:CFP-NTR)S891 [Transgenic: need MTA]
TgBAC(cyp1a:nls-egfp)cms2Tg [Transgenic: need MTA]

Zebrafish Mutant Line Genotyping

Genotyping mutant zebrafish lines to identify homozygous population. Certain mutant line requires more skilled labor than others to identify this population. This fee does not include husbandry and applies to all mutant zebrafish lines, regardless where housed in SARL. Addt'l fee of $1,500 for line maintenance up to 1 year and/or propogating next generation for $1,200.

$3,000.00 Item #

Assays

Zebrafish Developmental Toxicity Screen

The cost of evaluating for bioactivity potential. This includes two rounds of testing: 1 - dose range finding, 2 - definitive testing [also sold a la carte]

$4,000.00

Item #	Quantity
14534

Zebrafish Adult Behavior Assays

Conducting behavior assays on adult zebrafish. This for one of the following assays: free swim, predator, shoaling, and learning and memory. A minimum of 2 is required to account for controls.

$1,000.00 QuantityAssaysItem #

Service

PROPRIETARY DIET

Aquatic animals diet made at Sinnhuber Aquatic Research Laboratory (SARL). Full range detail available at the SARL.

$1,170.00

	Quantity	Item #
Special Diet (includes control)

Item subtotal:

Zebrafish Cancer Therapeutic Injections

Injection of one cell type into 2 day old zebrafish yolk sac or vein (up to 50), place embryos into 96 well plates, and provide care for the larvae.

$1,000.00 QuantityItem #

Cleaning/Staging Zebrafish Embryos

Cleaning and staging zebrafish embryos (up to 1000) - includes cost of embryos and transport container: Additional cost for every additional 1000, $40.

$75.00 Quantity

RNA/DNA Extraction of 1-96 Samples

This service will provide sample tissues in appropriate microtubes or 96 well plates in RNAshield for extraction. Cost is per sample up to 96 per run. Price includes supplies, reagents, and labor to extract using the Kingfisher Apex.

$18.00 Number of SamplesItem #

Quantification of Nucleic Acids

Quantification of dsDNA using BioTek Take3 microplate reader using UV spectrophotometry from samples provided in appropriate microtubes and in water. Price is for 1-8 samples.

$10.00 QuantityItem #

Bioinformatic Support

Bioinformatic support that includes consulting, designing, and analysis. A minimum of one hour will be charged. No written report or documentation is included. This is the hourly rate.

$100.00 QuantityItem #

Study Report Generation

Generating a study level report incorporating multiple services completed at SARL. Base fee is $400 per report (3 hours of work). Any additional time will be billed at $100 per hour per report.

$400.00 Item #Quantity

RNA/DNA Extraction

User of this service will provide sample tissues in appropriate microtubes in for nucleic acid extraction. Cost is per sample up to 18 per run. Price includes supplies, reagents, and labor.

$18.00 Item #

Zebrafish Injections

Injections of zebrafish (various life stages) up to 500 embryos per session up to 2 hours. Cost is dependent on Zebrafish type. Additional cost $40: additional embryos, up to 500. Additional cost: extra injection time @ 30 min increments, $50.

$650.00 Item #

Hourly

Hourly SARL Staff Support

Staff Support for staging, set up and maintenance of lab. Cost is per hour

$70.00 QuantityItem #

Hourly Consultation

Consultation and support. Does not qualify for a discount.

$175.00 QuantityItem #

Microscope Usage

Hourly rate to use microscope. Price varies on technology of microscope: Fluorescent Olympus $14, Keyence $70, Laser Dissection $48 , and Andor BC43 $80. Fee takes into consideration staff time for clean up, calibration and QC of equipment

$14.00 MicroscopesQuantityItem #

Shipping Supplies & Misc

Shipping, Supplies and Handling* [Required for Delivery] - Item # 10512

This includes all the necessary items to ship the order safely. Minimum quantity of 2.

$200.00 Quantity

International Handling Fee [Required Add On]

This fee covers the charges associated with the necessary paperwork and endorsements. This is required for all international shipments

$500.00

Enter coupon

Total

For questions, please contact:

Lisa Truong | Lisa.truong@oregonstate.edu
Should be Empty:

Entity	Discount (%)	Discount (code)
OSU / EHSC	75	OSU
Academia	50	ZF
Startup*	30	PER30OFF

Oregon State University - SARL

Order Placement*

For questions, please contact:

Order Placement